Biology Reference
In-Depth Information
CONCLUDING COMMENTS
Plants are valuable members of our global ecosystem and many scientists are ac-
tively working to understand the biology of these important organisms. Microtubules
and MAPs are the subject of much research because they play important roles in plant
growth and development. The ability to observe proteins tagged with fluorescent
proteins in intact living plants with confocal microscopes has helped the field
progress tremendously. The guidelines here describe how to generate and image
A. thaliana
plants expressing fluorescent fusion proteins.
Acknowledgment
I am grateful for support from Drs Michelle Bezanson and Claire Walczak.
References
Clough, S. J., Bent, A. F. (1998). Floral dip: a simplified method for Agrobacterium-mediated
transformation of Arabidopsis thaliana.
The Plant Journal
,
16
, 735-743.