Biology Reference
In-Depth Information
Table 12.1
Effects of Gs
α
QL, and Gs
α
peptide on microtubule dynamic instability
Dynamic instability
parameters
GsaQL
(2 mm)
Gsa peptide
(20 mm)
Control
0.1
a
0.3
a
Growing rate (mm/min)
1.6
0.1
2.6
2.6
1.3
a
1
a
Shortening rate (mm/min)
8.9
0.7
12.4
13.6
Time growing (%)
39
35
21.5
Time shortening (%)
11
23
15.6
Time attenuated (%)
50
42
62.9
0.02
a
0.1
b
Catastrophe frequency
(per min)
0.26
0.02
0.45
0.75
0.1
c
Rescue frequency (per min)
1.42
0.2
0.98
1.36
0.3
Dynamicity
1.44
2.35
2.42
Microtubules were polymerized to steady state at the ends of axoneme seeds in the absence and
presence of Gs
α
QL or Gs
α
peptide, and the dynamic instability parameters were determined. Data are
mean
S.E.
a
p
<
0.01 as observed in a t test with respect to control.
b
p
<
0.001 as observed in a t test with respect to control.
c
p
<
0.05 as observed in a t test with respect to control.
This research was originally published by
Dave et al. (2011)
.
FIGURE 12.5
Flowchart illustrating the steps in the cellular functional assays including Gsa-tubulin
interaction, microtubule dynamics in cells, and morphological assays.
