Biology Reference
In-Depth Information
The morphology of the mitochondrial network is not only changing during the
cell cycle, but actually has a crucial role during at least the G
1
to S transition
(
Mitra, Wunder, Roysam, Lin, & Lippincott-Schwartz, 2009
). The visualization
of the mitochondria in cells spread on micropatterns could help deepening the under-
standing of how and why their morphology correlates with or even causes the tran-
sitions in the cell cycle.
In summary, this method is useful whenever it is important to clearly see and
measure mitochondria morphology, localization, and dynamics in cells, fixed or liv-
ing, under controlled spatial conditions.
Acknowledgments
We thank Nicolas Carpi, Kristin Schauer, and Julie Janvore for helpful technical suggestions.
This work was supported by grants from ANR and LaLigue.
References
