Biology Reference
In-Depth Information
Whatman paper
parafilm
PLL(20)-g[3.5]-PEG(2), stock solution of 1 mg/ml in 10 mM Hepes, pH 7.4
(Surface Solutions GmbH, Switzerland)
10 mM HEPES, pH 7.4
ddH
2
O
KimWipes
Plasma cleaner (Harrick Plasma)
synthetic quartz mask with features (Toppan photomasks)
UV lamp, 185 nm (Jelight, UVO Cleaner 42) with an ozone killer (or under
a hood).
7.5.2
Seeding and spreading of cell
fibronectin (Sigma, F1141)
fluorescently labeled fibrinogen, resuspended to 1 mg/ml (Invitrogen, e.g.,
F-13191)
complete culture medium:
DMEM/F-12 (Invitrogen/Gibco, 21041) complemented with
1-10% fetal bovine serum (Biowest, S1810)
2mM
L
-glutamine (Invitrogen/Gibco, 25030)
1% penicillin/streptomycin (Invitrogen/Gibco, 15140)
2 g/l sodium bicarbonate (Invitrogen/Gibco, 25080) (for CO
2
atmosphere)
culture flasks and dishes (TPP)
PBS
TrypLE (Invitrogen/Gibco, 12605)
coverslips (round or square, fitting the live-cell chamber)
PAF (for fixing)
slides
7.5.3
Mitochondria and microtubule visualization
MitoTracker
®
Probes (Invitrogen, M7512, M22426, etc.)
fluorescent protein Mito (derived from pEYFP-Mito vector, Clontech, ref. 6115-1)
selection antibiotics for stable cell line
cell culture medium
chamber for live-cell visualization (magnetic from Live Cell Instrument or
Cytoo, or Ludin from Life Imaging Services)
Blocking/antibody dilution buffer (PBSAT):
PBS
1% BSA fraction V (Sigma, A3059)
0.5% Triton X-100 (dilute from 25% stock)
Microtubule antibody (e.g., anti-
a
-tubulin B-5-1-2, Sigma) diluted in PBSAT
Fluorescent secondary antibody diluted in PBSAT
DAPI 1 mg/ml (dilute in H
2
O from 5000
stock)
