Biology Reference
In-Depth Information
A
Mitochondria
Tubulin
Merge + nucleus
Tubulin
B
10
m
m
FIGURE 7.2
Micropatterning technique is robust. (A) A comparison between RPE1 cells seeded onto
nonpatterned surface and cells seeded onto rectangular patterned surface. Nonpatterned
cells can adopt many shapes, making analysis of dynamics subcellular structures such
as mitochondria and microtubules challenging. Bar 10
m. (B) Patterned shapes are
repeatable, given rise to standardized cell shapes and size, making analysis of dynamics
subcellular structures such as mitochondria and microtubules less challenging.
m
7.5
MATERIALS
7.5.1
Preparation of micropatterns
glass coverslips (suitable size for the live-cell chamber)
70% EtOH
large Petri dish
