Biomedical Engineering Reference
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avoid unnecessary heating. There are two filters in the cube. The excitation filter allows wavelengths
smaller than the emitted fluorescence wavelengths, while the emission filter allows through only the
longer fluorescence wavelengths. Neutral density filters are the most common types of filters used in
a fluorescence microscope. Neutral density filters are usually fabricated by evaporation of a thin metal
layer onto the glass surface. A fluorescence microscope can be equipped with different optical filter
sets. Common fluorescent dyes have fixed wavelength pairs and need a corresponding filter set. Filter
FIGURE 8.4
Fluorescence microscope: (a) schematic concept and (b) transmission characteristics.
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