Biomedical Engineering Reference
In-Depth Information
Modulating Extracellular Matrix
at Interfaces of Polymeric Materials
Carsten Werner (
)·TiloPompe·KatrinSalchert
Leibniz Institute of Polymer Research Dresden, Max Bergmann Center of Biomaterials,
Hohe Str. 06, 01069 Dresden, Germany
werner@ipfdd.de
1
I tr cti n ...................................
64
2
Biospecific and Physical Characteristics of Extracellular Matrices .....
67
3
Reconstitution of Extracellular Matrix Assemblies in vitro .........
71
4
Matrix Assembly and Functionality at Polymer Surfaces
..........
76
4.1
Fibronectin Anchorage to Polymer Films Directs Fibrillogenesis
byandAngiogenesisofEndothelialCells ...................
78
4.2
Collagen Fibrils and Collagen-Glycosaminoglycan Cofibrils
on Planar Surfaces and in 3D Carrier Materials
toImitatetheHematopoieticNiche ......................
82
5
Perspective: Bio-Hybrid Extracellular Matrices ................
88
References
.......................................
89
Abstract As extracellular matrices (ECM) closely interact with cells in living tissues and,
through this, influence essentially any aspect of life engineering of ECM currently receives
a lot of attention in the advent of regenerative therapies. Artificial matrices based on
biopolymers isolated from nature were successfully utilized to prepare various types of
cell scaffolds to enhance the integration and performance of engineered tissues. Beyond
that, translation of progress in matrix and cell biology into new concepts of materi-
als science permits to further refine the functional characteristics of such reconstituted
matrices to direct tissue regeneration processes. The review emphasizes research to mod-
ulate the functionality of ECM biopolymers through their combination with synthetic
polymeric materials. Two examples referring to our own studies concern (1) the control
of vasculogenesis by adjusting the availability of surface bound fibronectin for cell-driven
reorganization and; (2) the imitation of the bone marrow niche with respect to the cul-
tural amplification of hematopoietic progenitor cells using collagen I-based assemblies. As
a perspective we briefly discuss the design of biohybrid ECM mimics where synthetic and
natural polymers are combined on the molecular scale for future use as morphogenetic
templates in in vivo tissue engineering applications.
Keywords Extracellular matrix · Fibronectin · Collagen · Reconstitution
 
 
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