Biomedical Engineering Reference
In-Depth Information
the cellular coagulation (activation of thrombocytes with release of platelet
factor 4 or
-thromboglobulin) and plasmatic coagulation (release of pro-
thrombin fragment F1+2) was observed, the latter, however, only to a small
extent [163]. The complement activation of human serum due to P3HB
contact was demonstrated by a significant increase of the C3a-desArg con-
centration [164]. However, no significant activation of the coagulation and
complement systems by P3HB films have been observed in subsequent studies
(Fig. 8) [72]. The conflicting results might be attributed to different degrees in
the purity of the polymer used in both sets of experiments.
TheimportanceofpolymerpurityonthebloodcompatibilityofP3HB
and P3HB-3HV (4%, 18% 3HV) has recently been investigated. It has been
β
Fig. 8
In vitro hemocompatibility of solution-cast P3HB films, compared to that of P3HB
films modified with 30% each of at-P3HB, dg-P3HB, TEC (leached), and BTHC; PLLA
films for comparison. Activation of
a
cellular coagulation (release of
-thromboglobulin,
control = collagen),
b
plasmatic coagulation (release of prothrombin fragment F1+2, con-
trol = kaolinite), and
c
complement system (release of C3a-desArg, control = inulin) [72]
β
