Interfaces to Control Cell-Biomaterial Adhesive Interactions - Advances in Polymer Science - page 171

Interfaces
to Control Cell-Biomaterial Adhesive Interactions

Andrés J. García
Woodruff School of Mechanical Engineering, Petit Institute for Bioengineering
and Bioscience, Georgia Institute of Technology, 315 Ferst Drive, IBB 2314,
Atlanta, GA 30332-0363, USA
[e-mail]

1 Cell Adhesion .................................. 172

1.1 SignificanceofCellAdhesion.......................... 172

1.2 IntegrinAdhesionReceptors .......................... 172

1.3 Adhesive Interactions in Cell and Host Responses to Biomaterials . . . . . 175

2 Surfaces Controlling Protein Adsorption and Activity ............ 176

2.1 ProteinAdsorptioninCell-BiomaterialInteractions ............. 176

2.2 SurfacesThatResistProteinAdsorption.................... 177

2.3 SubstratesModulatingAdsorbedProteinActivity .............. 178

3 Biomimetic Interfaces Promoting Cell Adhesion ............... 180

3.1 BiologicalMotifsasTargetsforBiomaterialApplications .......... 180

3.2 First-Generation Biomimetic Adhesive Supports: Short Oligopeptides . . . 180

3.3 Second-Generation Biomimetic Adhesive Supports:

LigandswithIntegrinSpecificity........................

182

4 Micropatterned Supports to Control Cell Adhesion ............. 185

4.1 EngineeringCellShapeandAdhesiveArea .................. 185

4.2 AdhesionStrengtheningResponsestoMicropatternedSurfaces....... 186

5 Conclusions and Future Prospects ....................... 187

References ....................................... 188

Abstract Cell adhesion to adsorbed proteins and adhesive sequences engineered on sur-
faces is crucial to cellular and host responses to implanted devices, biological integration
of biomaterials and tissue-engineered constructs, and the performance of biosensors,
cell-based arrays, and biotechnological cell-culture supports. This review focuses on
interfaces controlling cell-adhesive interactions, with particular emphasis on surfaces
controlling protein adsorption, biomimetic substrates presenting bioadhesive motifs, and
micropatterned surfaces to engineer adhesive areas. These approaches represent promis-
ing strategies to engineer cell-material biomolecular interactions in order to elicit specific
cellular responses and enhance the biological performance of materials in biomedical and
biotechnological applications.

Keywords Cell adhesion · Collagen · Fibronectin · Focal adhesions · Integrins