Multimodal Biomedical Imaging Systems - Biomedical Optical Imaging Technologies: Design and Applications - page 314

Fig. 9.7 Configurations of
multimodal imaging system
combining polarized
reflectance, fluorescence, and
OCT imaging modalities.
( a ) Three modalities share the
objective lens, and ( b ) only
polarized reflectance and
fluorescence imaging use the
same imaging lens

9.3.2 Fluorescence Imaging

As shown in Fig. 9.8 , most fluorescence imaging systems have the following key
elements: light source to excite fluorescence, illumination optics to deliver the
excitation light, light collection or imaging optics to collect the fluorescence signal,
fluorescence filters, and a detector to capture the fluorescence signal. Fluorescence
filters generally include excitation filter, emission filter, and dichroic beamsplitter.
An effective fluorescence imaging system requires careful consideration of the
illuminating light source, excitation/emission filters, and detector, as well as the
illumination and detection optics because the fluorescence signal is typically very
weak. In this section, these components will be discussed in detail.