Biomedical Engineering Reference
In-Depth Information
Fig. 6.6
Axial images of a
thin film of SiO
2
on a silicon
substrate, recorded using an
NA of 0.8 and HeNe laser
. D 633 nm/:(
a
)
experimental result, (
b
)
theoretical prediction, (
c
)
experimental confocal
interference image
homodyne system, where fringe information can be used as a measure of image
phase (Fig.
6.6
c).
The confocal microscope also has some other advantages. The pinhole, as well as
rejecting out-of-focus information, also rejects stray light, so that images have a high
signal-to-noise ratio. The rejection of stray light also gets rid of scattered light, thus
allowing penetration into a scattering medium such as biological tissue. In addition,
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