Spectral Imaging: Methods, Design, and Applications - Biomedical Optical Imaging Technologies: Design and Applications - page 154

Fig. 4.21 a Spectra of the five fluorochromes that are used for SKY (FITC, Rhodamine, Texas
Red, Cy5, and Cy5.5 or alike). b The spectrum measured for each one of the labeled chromosomes.
Spectra are measured with the SpectraCube system through a triple dichroic SKY filter cube with
a spectral resolution of 10 nm at 500 nm

were stained with only a single stain. By correlating the single-stain distribution, it
also provides information on the co-localization of structures, proteins, and other