Fluorescence Microscopy Imaging in Biomedical Sciences - Biomedical Optical Imaging Technologies: Design and Applications - page 101

resolution. The popularity of these fluorescence microscopy techniques will soon
spread by the development of turn-key instruments, improvements of user-friendly
data acquisition and analysis software, and demonstration of their unique usefulness
to biological applications, many of which have already taken place.
Acknowledgements The authors greatly acknowledge the finance support provided by the
National Center for Research Resources (NCRR) - National Institutes of Health (NIH) RR025616,
RR027409, P01HL101871 and the University of Virginia. The authors would like to thank
Mr. Horst Wallrabe for his critical reading of our manuscript and helpful suggestions.
References
1. A.H. Coons, H.J. Creech, R.N. Jones, E. Berliner, The demonstration of pneumococcal
antigen in tissues by the use of fluorescent antibody. J. Immunol. 45 (3), 159-70 (1942)
2. O. Shimomura, F.H. Johnson, Y. Saiga, Extraction, purification and properties of aequorin, a
bioluminescent protein from the luminous hydromedusan, aequorea. J. Cell Comp. Physiol.
59 , 223-39 (1962)
3. D.C. Prasher, V.K. Eckenrode, W.W. Ward, F.G. Prendergast, M.J. Cormier, Primary structure
of the aequorea victoria green-fluorescent protein. Gene 111 (2), 229-33 (1992)
4. M. Chalfie, Y. Tu, G. Euskirchen, W.W. Ward, D.C. Prasher, Green fluorescent protein as a
marker for gene expression. Science 263 (5148), 802-805 (1994)
5. R.Y. Tsien, The green fluorescent protein. Annu. Rev. Biochem. 67 , 509-544 (1998)
6. N.C. Shaner, P.A. Steinbach, R.Y. Tsien, A guide to choosing fluorescent proteins. Nat.
Methods 2 (12), 905-909 (2005)
7. R.N. Day, M.W. Davidson, The fluorescent protein palette: Tools for cellular imaging. Chem.
Soc. Rev. 38 (10), 2887-2921 (2009)
8. D.J. Stephens, V.J. Allan, Light microscopy techniques for live cell imaging. Science
300 (5616), 82-86 (2003)
9. W.A. Hing, C.A. Poole, C.G. Jensen, M. Watson, An integrated environmental perfusion
chamber and heating system for long-term, high resolution imaging of living cells. J. Microsc.
199 (Pt 2), 90-95 (2000)
10. C.L. Rieder, A. Khodjakov, Mitosis through the microscope: Advances in seeing inside live
dividing cells. Science 300 (5616), 91-96 (2003)
11. P.J. Davis, E.A. Kosmacek, Y. Sun, F. Ianzini, M.A. Mackey, The large-scale digital cell
analysis system: An open system for nonperturbing live cell imaging. J. Microsc. 228 (Pt 3),
296-308 (2007)
12. P. Gross, G. Farge, E.J. Peterman, G.J. Wuite, Combining optical tweezers, single-molecule
fluorescence microscopy, and microfluidics for studies of DNA-protein interactions. Methods
Enzymol. 475 , 427-453 (2010)
13. J.R. Lakowicz, Principles of Fluorescence Spectroscopy (Springer, New York, 2006)
14. T. Nagai, K. Ibata, E.S. Park, M. Kubota, K. Mikoshiba, A. Miyawaki, A variant of yellow
fluorescent protein with fast and efficient maturation for cell-biological applications. Nat.
Biotechnol. 20 (1), 87-90 (2002)
15. G.H. Patterson, J. Lippincott-Schwartz, A photoactivatable GFP for selective photolabeling
of proteins and cells. Science 297 (5588), 1873-1877 (2002)
16. J. Zhang, R.E. Campbell, A.Y. Ting, R.Y. Tsien, Creating new fluorescent probes for cell
biology. Nat. Rev. Mol. Cell Biol. 3 (12), 906-918 (2002)
17. J. Lippincott-Schwartz, G.H. Patterson, Development and use of fluorescent protein markers
in living cells. Science 300 (5616), 87-91 (2003)