Biomedical Engineering Reference
In-Depth Information
Figure 10.19
Reduction in signal height of the DnaJ(1-70) NH TROSY-HSQC cross
peaks due to the presence of spin-labelled DnaK. (a) DnaK(1-605)V210C-
MTSL and NR (DnaJ:DnaK ratio: blue, 1 : 0; green, 1 : 0.5; red, 1 : 1.2).
(b) As above, but with DnaK(1-605)D326C-MTSL and NR (DnaJ:DnaK
ratio: blue, 1 : 0; red, 1 : 1.2). (c) With DnaK(1-605)K421C-MTSL and
NR (DnaJ:DnaK ratio: blue, 1 : 0; red, 1 : 1.2). Adapted from ref. 32.
