Biomedical Engineering Reference
In-Depth Information
principles that apply widely across a wide range of RNA structures. In particular,
a great deal of information has been obtained regarding the dependence of inter-
helical motions on RNA secondary structure as well as external factors suchas
ligands and metals. The generality of these findings has to be examined by
investigating structurally and functionally distinct RNA molecules—including
RNA containing three-way and higher order junctions. Studies must also target
larger more complex RNA architectures and explore other modes of motion,
such as base-flipping and changes in hydrogen bond alignments. We hope that
this chapter will help enable some of these future applications.
Acknowledgements
We thank members of the Al-Hashimi lab for insightful comments and Dr.
Alex Kurochkin for his expertise and maintenance of the NMR instruments.
H. M. A. would like to acknowledge fruitful collaborations with the groups of
Carol Fierke (University of Michigan), Charles L. Brooks III (University of
Michigan), and Ioan Andricioaei (University of California - Irvine). The
authors gratefully acknowledge the Michigan Economic Development
Cooperation and the Michigan Technology Tri-Corridor for support in the
purchase of the 600 MHz spectrometer. This work was supported by the
National Institutes of Health (RO1 AI066975-01 and RO1-GM089846) and a
National Science Foundation CAREER award (MCB 0644278).
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