Biomedical Engineering Reference
In-Depth Information
Figure
3.4.
Purified P. pastoris-expressed EBA-175 RII (A) or RII-NG (B) protein as detected by
Coomassie blue-stained SDS-PAGE gel analysis. Purified recombinant proteins were separated
using a 4-20% gradient gel under nonreducing conditions.
Figure
3.5.
Space-filling representation of the dimeric structure of P. falciparum EBA-175 RII [47]
showing positions of amino acid substitutions for expression of RII-NG. Monomers are shaded in
cyanoblue and gray. The four amino acid substitutions made, which can be identified in the crystal
structure, are shown in red. (See the insert for color representation of this figure.)
