Biomedical Engineering Reference
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Figure 3.2. Nucleotide sequence alignment for native P. falciparum 3D7 EBA-175 RII (nRII) and
synthetic mammalian codon optimized RII-NG (sRII).
quantity and quality attributes. At the time, the baculovirus expression system was not
considered suitable for scalable production, for reasons stated above. The P. pastoris
expression system was of particular interest due to its scalability, capacity for posttrans-
lational modifications (higher probability of correct disulfide bond formation), and ease
of purification of a secreted product.
In the late 1990s, expression projects were being developed around the use of highly
expressed genes with bias toward codons that are recognized by the most abundant tRNA
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