Computational Modeling of Mass Transport and Its Relation to Cell Behavior in Tissue Engineering Constructs - Computational Modeling in Tissue Engineering - page 90

Fig. 3 a LIVE/DEAD viability images of human periosteum derived cells cultured for 14 days
in fibrin hydrogels (bar = 1 mm). b H & E staining on histological sections of a fibrin construct
cultured for 21 days (bar is 1 mm for the left image and 100 lm in the two images at higher
magnification). Figure reprinted from [ 25 ]
could originate from differences in molecular size as described in previous sec-
tions, but could also be attributed to differences in cellular response, which will be
described in the next sections.
Another strategy aiming to overcome these limitations of inadequate solute
mass transport is to induce de novo synthesis of a vascular network or sprouting of
existing vessels using the body as an in vivo bioreactor system [ 116 ]. The use of
mathematical models has been proposed in this context as a tool for the intelligent
design of scaffold structures and implantation techniques [ 22 ]. The experimental in
vivo model investigated by these authors consisted of hepatocyte-seeded PLGA