Oxygen Transport in Bioreactors for Engineered Vascular Tissues - Computational Modeling in Tissue Engineering - page 281

Table 1 Parameters used in DO and glucose transport models
Parameter Description Value Source

Tissue properties
L Axial length 5 cm Measured

H Tissue thickness 300 lm Measured

R i Inner diameter 2 mm Measured

R o Outer diameter 2.3 mm Measured

1 9 10 -6 cm s -1 Pa -1 L p Hydraulic conductivity Measured

250 9 10 6 cells mL -1

q cell Cell density Measured

V max ; O 2 Michaelis-Menten parameter for O 2 1.66 fmol/min/cell Measured

K m ; O 2 Michaelis-Menten parameter for O 2 15.8 lM Measured

V max, gluc Michaelis-Menten parameter for glucose 9.25 fmol/min/cell [ 30 ]

K m, gluc Michaelis-Menten parameter for glucose 1.5 mM [ 31 ]

1.06 9 10 -5 cm 2 s -1 D O 2 ; t Effective O 2 diffusivity in tissue [ 33 ]

1.6 9 10 -7 cm 2 s -1

D gluc, t Effective glucose diffusivity in tissue [ 34 ]

Fluid properties
q 0.992 g cm -3 Density (water), 37C [ 35 ]

0.0069 g cm -1 s -1 l Dynamic viscosity (water) [ 35 ]

3.83 9 10 -5 cm 2 s -1 D f Effective O 2 diffusivity in culture medium [ 36 ]

177 (140) nmol mL -1

C ? Equilibrium DO concentration (oxygen tension) (mmHg) Measured

C 0, gluc Culture medium glucose concentration 25 mM [ 37 ]

x Pulse frequency 0.5-1 Hz Measured

V s Stroke volume 0.5-1 mL Measured