Biomedical Engineering Reference
In-Depth Information
Chapter 4
Lensfree Computational Microscopy Tools
for On-Chip Imaging of Biochips
Serhan O. Isikman, Waheb Bishara, Onur Mudanyali, Ting-Wei Su,
Derek Tseng, and Aydogan Ozcan
Abstract
The use of optical imaging for medical diagnostics at the point of care
(POC) has great potential, but is limited by cost and the need for highly trained
personnel. To this end, the cost, complexity, and size of optical microscopy devices
can be reduced through the use of computation. These techniques can perform
particularly well at specific tasks such as cytometry, water quality management, and
disease diagnostics. This chapter focuses on lensfree on-chip imaging techniques
that are based on partially coherent digital in-line holography and are especially
promising for imaging of biochips toward field-use and telemedicine applications.
This emerging imaging platform discards most optical components that are found
in traditional microscopes such as lenses and compensates for the lack of physical
components in the digital domain. Widely available image sensors and abundant
computational power are used to digitally process the acquired raw data to recover
traditional microscope-like images with submicron resolution over large sample
volumes within biochips.
4.1
Introduction
Optical imaging has been a cornerstone of science and medicine for centuries.
Despite the recent developments that optical imaging has witnessed in terms of
spatial resolution, speed, sensitivity, throughput, and other performance metrics
A. Ozcan (
)
Electrical Engineering Department, University of California, Los Angeles, USA
California NanoSystems Institute, Bioengineering Department, Department of Surgery,
University of California, Los Angeles, USA
e-mail:
ozcan@ee.ucla.edu
S.O. Isikman
W. Bi shara
O. Mudanyali
T.-Wei Su
D. Tseng
Electrical Engineering Department, University of California, Los Angeles, USA
