Biomedical Engineering Reference
In-Depth Information
Chapter 9
Diagnostic Magnetic Resonance Technology
Changwook Min, Huilin Shao, David Issadore, Monty Liong,
Ralph Weissleder, and Hakho Lee
Abstract
For the sensitive and quantitative measurement of protein biomarkers,
pathogens, and cells in clinical samples, magnetic nanoparticles (MNPs) offer
unique advantages over traditional detection methods. Specifically, due to the
inherently negligible magnetic background of biological material, MNPs can be
used to obtain highly sensitive measurements in minimally processed samples.
Our detection platform, termed diagnostic magnetic resonance (DMR), exploits
MNPs to modulate the nuclear magnetic spin-spin relaxation time of water. Here,
we review work done by our group to develop more effective MNP biosensors,
advanced conjugational strategies to target the MNPs to molecular targets, and
highly sensitive miniaturized NMR systems. We demonstrate this platform as a
robust and easy-to-use system for the detection of a wide range of targets in clinical
settings including whole cells, proteins, DNA/mRNA, metabolites, drugs, viruses,
and bacteria.
C. Min
H. Lee (
)
Center for Systems Biology, Massachusetts General Hospital, 185 Cambridge St, CPZN 5206,
Boston, MA 02114, USA
e-mail:
lee@mgh.harvard.edu
R. Weissleder
Center for Systems Biology, Massachusetts General Hospital, 185 Cambridge St, CPZN 5206,
Boston, MA 02114, USA
Department of Systems Biology, Harvard Medical School, 200 Longwood Ave, Boston,
MA 02115, USA
D. Issadore
H. Shao
M. Liong
Bioengineering, University of Pennsylvania, Philadelphia, PA, USA
