Biomedical Engineering Reference
In-Depth Information
Fig. 7.15 ( a ) Real-time binding curves of diluted HCV capsid protein at concentrations ranging
from 1,000 ng/mL to 100 pg/mL. The sensors, functionalized with bovine serum albumin (BSA) as
a negative control, gave minimal signal indicating negligible non-specific binding of the wash-free
assay. ( b ) Calibration curves for each marker of interest were generated after 15 min of incubation
time on a log-log scale [ 17 ]
Tabl e 7. 1 The itemized cost of the one-time use disposable stick, including the circuit
board, sensors, capture and detection antibodies for eight sensors, magnetic nanopar-
ticles, surface chemistry, and assembly, is shown below. The volume of production is
calculated for one million units per year. It is further assumed that the antibodies are
robotically spotted to reduce the required volume and assembly cost
Item
Unit cost ($)
Circuit board and connector
0.34
GMR sensor die
1.00
HCV capture antibody (abcam 2583)
0.03
HCV detection antibody (abcam 58713)
0.03
Magnetic nanoparticles (Miltenyi Biotec 130-048-101)
1.88
Surface chemistry reagents
0.01
Assembly
0.20
Total
3. 49
7.7
Conclusions
The handheld device described in this chapter has the potential to provide a
significant contribution to the future of POC medical diagnostics. As the population
continues to expand and societal mandates for universal healthcare grow, innova-
tions in diagnostic testing will be required to provide timely, easily accessible, and
inexpensive results. To meet this need, it is necessary to develop cost-effective,
portable, and easy-to-use devices which allow individuals to conduct their own
molecular diagnostic tests without the need for a centralized laboratory, laboratory
technicians, clinic or emergency room visits, or in some instances visits to a
physician's office.
 
Search WWH ::




Custom Search