Biology Reference
In-Depth Information
CHAPTER
C. elegans, and Mammals
1
Huimin Na
*
,{
, Peng Zhang
*
,{
, Yunfeng Ding
*
, Li Yang
*
,{
, Yang Wang
*
,{
, Huina
Zhang
*
, Zhensheng Xie
*
, Fuquan Yang
*
, Simon Cichello
{
,
and Pingsheng Liu
*
*
National Laboratory of Biomacromolecules, Institute of Biophysics, Chinese Academy of Sciences,
Beijing, China
{
University of Chinese Academy of Sciences, Beijing, China
{
School of Life Sciences, La Trobe University, Melbourne, Victoria, Australia
Proteomic Studies of Isolated
Lipid Droplets from Bacteria,
CHAPTER OUTLINE
Introduction ................................................................................................................ 2
1.1 Equipment ........................................................................................................... 3
1.2 Reagents ............................................................................................................. 3
1.3 Methods .............................................................................................................. 4
1.4 Discussion......................................................................................................... 10
Acknowledgment....................................................................................................... 12
References ............................................................................................................... 12
Abstract
Lipid droplets (LDs) are an intracellular organelle, consisting of a neutral lipid core
covered by a monolayer of phospholipids and proteins. It primarily mediates lipid
storage, metabolism, and transportation. Recently, research of LDs has emerged
as a rapidly developing field due to the strong linkage between ectopic lipid accu-
mulation and metabolic syndromes. Recently, more than 30 proteomic studies of iso-
lated LDs have identified many important LD proteins that have highlighted and
have also predicted the potential biological roles of the organelle, motivating the
field to develop quite rapidly. This chapter summarizes methods used in proteomic
studies for three representative species reported and discusses their advantages and
disadvantages. We believe that this chapter provides useful information and methods
for future LD proteomic studies especially for LDs in other species.
