Biology Reference
In-Depth Information
FIGURE 5.2
Putative mutants with changes in oil contents or oil droplet morphology. (A) A thin-layer
chromatogram of lipid extracts is shown. Algal cells were grown in Tris-acetate-phosphate
(TAP) medium lacking NH
4
for 3 days. Mutants with increased or decreased oil accumulation
are indicated by red or black asterisks, respectively. Lipids were loaded on an equal-cell-
number basis. Oils were visualized by iodine staining. Soy oil was included as a standard. (B)
Nile red staining of Chlamydomonas cells cultured for 1 week in media lacking NH
4
þ
. Note an
increase in size but decrease in number of oil droplets in the mutant line 15-66.
Bars
¼
10
m.
m
