Biology Reference
In-Depth Information
CHAPTER
3
Visualization of Lipid
Droplets in C. elegans by
Light and Electron
Microscopy
Ho Yi Mak
*
,{
*
Stowers Institute for Medical Research, Kansas City, Missouri, USA
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Department of Molecular and Integrative Physiology, University of Kansas Medical Center,
Kansas City, Kansas, USA
CHAPTER OUTLINE
Introduction .............................................................................................................. 40
3.1 Transgenic Expression of Lipid Droplet Markers in C. elegans .............................. 41
3.2 Visualization of Lipid Droplet Associated Proteins by Laser Scanning Confocal
Microscopy ....................................................................................................... 43
3.2.1 Background ..................................................................................... 43
3.2.2 Methods .......................................................................................... 43
3.2.3 Discussion ....................................................................................... 44
3.3 Visualization of Lipid Droplet Associated Proteins by Spinning Disk Confocal
Microscopy ....................................................................................................... 45
3.3.1 Background ..................................................................................... 45
3.3.2 Methods .......................................................................................... 45
3.3.3 Discussion ....................................................................................... 45
3.4 Visualization of Lipid Droplets by TEM ................................................................ 46
3.4.1 Background ..................................................................................... 46
3.4.2 Methods .......................................................................................... 46
3.5 Visualization of Lipid Droplet Associated Proteins by Immunoelectron
Microscopy ....................................................................................................... 47
3.5.1 Background ..................................................................................... 47
3.5.2 Methods .......................................................................................... 47
Summary .................................................................................................................. 48
Acknowledgments ..................................................................................................... 48
References ............................................................................................................... 48
