Biology Reference
In-Depth Information
Before bleach
After bleach
2.2 min recovery
Before bleach
After bleach
19 s recovery
Final
2.0
***
1.5
1.0
0.5
0.20
120%
120%
0.15
Bleached
Unbleached
Bleached
Unbleached
0.10
100%
100%
0.05
80%
80%
0.00
60%
60%
40%
40%
20%
20%
0%
0%
-
1
0
1
2
3
-
2
0
2
4
6
Time (min)
Time (min)
FIGURE 14.2
Plin1 accelerates Fsp27-mediated neutral lipid exchange when coexpressed in 3T3-L1 preadipocytes. Neutral lipid signal (red) in one LD was
bleached (white circle, upper panel). Bars, 2
m. MOI in bleached region (white circle) and unbleached region (blue circle) were plotted as
percentage of the initial intensity from four independent experiments (mean
m
SD, lower panel). Arrowheads point to the starting time of
each bleaching in repeated FRAPs (mid-low panel). Right panel: the neutral lipid exchange rate in 3T3-L1 preadipocytes expressing Fsp27-GFP
alone or coexpressing with Plin1. Data were collected from five independent experiments (***p
<
0.001).
