Biology Reference
In-Depth Information
GFP
Bodipy 558/568
DAPI
Merge
GFP control
Fsp27-GFP
Fsp27-GFP
+
HA-Plin1
LD size distribution
60.00%
50.00%
Control
Fsp27-GFP
Fsp27+HA-Plin1
40.00%
30.00%
20.00%
10.00%
0.00%
0
0.5
1
1.5
2
2.5
3
3.5
4
4.5
5
5.5
6
6.5
Diameter /
m
m
FIGURE 14.1
Plin1 enhances Fsp27's activity in promoting large LD formation. Fsp27-GFP is coexpressed
with empty vector or HA-Plin1 in 3T3-L1 preadipocytes. Fsp27-GFP expression promotes
large LD formation in comparison with GFP control. Coexpressing Fsp27 with Plin1 further
increases LD sizes. The lower panel shows LD size distribution quantified from 30 cells. In
comparison with cells expressing Fsp27-GFP alone, cells coexpressing Fsp27 and Plin1
accumulate more LDs that have diameters larger than 3
m.
m
14.2.1
Materials and experimental procedures
14.2.1.1
Materials
3T3-L1 preadipocytes or differentiated 3T3-L1 adipocytes
35 mm Labtek live cell imaging culture chamber
