Biology Reference
In-Depth Information
CHAPTER
14
Zhiqi Sun, Jingyi Gong, Lizhen Wu, and Peng Li
Tsinghua-Peking Center for Life Sciences, School of Life Sciences, Tsinghua University,
Beijing, China
Imaging Lipid Droplet
Fusion and Growth
CHAPTER OUTLINE
Introduction ............................................................................................................ 254
Biogenesis and Cellular Functions of Lipid Droplets .................................. 254
Various Mechanisms of LD Growth ........................................................... 255
14.1 Quantitative Measurement of LD Size ...............................................................256
14.1.1 Measuring LD Sizes in 3T3-L1 Preadipocytes or Mature Adipocytes 256
14.1.1.1 Materials ........................................................................... 256
14.1.1.2 Experimental Procedure..................................................... 256
14.1.2 Data Analysis and Representative Results..................................... 257
14.2 Measuring Neutral Lipid Exchange Through FRAP..............................................257
14.2.1 Materials and Experimental Procedures ........................................ 258
14.2.1.1 Materials ........................................................................... 258
14.2.1.2 Experimental Procedures ................................................... 259
14.2.2 Data Analysis and Representative Results..................................... 261
14.2.2.1 Algorithm for the Calculation of Lipid Exchange Rate .......... 261
14.2.2.2 Representative Results ...................................................... 262
14.3 Measuring LD Fusion Rate ...............................................................................262
14.3.1 Materials and Experimental Procedures ........................................ 263
14.3.1.1 Materials ........................................................................... 263
14.3.1.2 Experimental Procedures ................................................... 263
14.3.2 Data Analysis and Representative Results..................................... 263
Conclusions............................................................................................................ 265
Acknowledgments ................................................................................................... 265
References ............................................................................................................. 265
Abstract
Lipid droplets (LDs) are highly dynamic cellular organelles found in most eukaryotic
cell types. In white adipocytes, LDs grow into a characteristic unilocular morphology
