Biology Reference
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FIGURE 12.1
Perilipin 3 falls along the endoplasmic reticulum (ER) at the cell periphery. OP9 cells were
incubated for 1 h with 1.8 mM of the fatty acid oleate bound to albumin at a ratio of 5:1. Cells
were fixed and stained for perilipin 3 at 1.6 m g/ml, and the ER was visualized by
immunostaining for transfected FLAG-DGAT1 as described previously ( Skinner et al., 2009 ).
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m in all figures.
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12.2.2.1 Cell growth and density
Cell density is also important. Cells with fibroblastic morphology are typically plated
at between 30% and 60% confluency (surface area covered), since they spread out
and form large flat processes that abut neighboring cells ( Figs. 12.1 and 12.3 ). As
cells proliferate, the cell layer thickens and overlapping structures make images dif-
ficult to interpret. At very low density (less than 10% confluent) it is difficult to
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