Biology Reference
In-Depth Information
3.
Gene Pulser II (Bio-Rad) for electroporation of hepatoma cells
4.
Gene Pulser cuvette, 0.4 cm electrode gap (Bio-Rad, 165-2088)
5.
Luciferase Assay System (Promega)
6.
MonoLight 2010 Luminometer (Pegasus Scientific Inc.)
7.
RiboMax T7 kit (Promega) with the 7mG(ppp)A RNA cap analog for
in vitro
transcription of DENV RNA
8.
0.8% methylcellulose as an overlay in the plaque assay
10.1.2
Tools for analysis of LDs
1.
Oleic acid-albumin from bovine serum (Sigma O3008)
2.
DGAT1 inhibitor (2-((1
s
,4
s
)-4-(4-(4-amino-7,7-dimethyl-7
H
-pyrimido[4,5-
b
]
[1,4]oxazin-6 yl)phenyl)cyclohexyl)acetic acid) (Syngene International)
3.
DGAT1 inhibitor 4a, (1
R
,2
R
)-2-[[4'-[[(phenylamino)carbonyl]amino]
[1,1'-biphenyl]-4-yl]carbonyl]cyclopentanecarboxylic acid (TOCRIS
Bioscience, A 922500)
4.
HCS LipidTOX Red and Far Red neutral lipid stain (Invitrogen)
5.
BODIPY 493/503 (Molecular Probes, D-2191)
6.
Oil-Red-O (ORO) (Sigma, O0625)
7.
Infinity triglycerides (Thermo Scientific, TR22421)
8.
HR Series NEFA-HR(2) (Wako, 999-34691, 991-34891, 993-35191, 276-
76491)
9.
Tight-fitting Dounce homogenizer (Wheaton)
10.
Triacsin C (Sigma, T4540)
10.1.3
Other commercial reagents and drugs
X-tremeGENE 9 DNA Transfection Reagent (Roche)
10.1.4
Other buffers and reagents
Crystal violet stain
:
Stock solution: 1 g crystal violet, 99 ml 20% EtOH
Working solution: 20 ml stock solution, 40 ml EtOH, 150 ml H
2
O
Hypotonic buffer
:
50 mM HEPES, pH 7.4
1 mM EDTA
2 mM MgCl
2
Isotonic buffer
:
50 mM HEPES, pH 7.4
100 mM KCl
2 mM MgCl
2
Sucrose isotonic buffer
:
Isotonic buffer containing either 1.5 or 2 M sucrose (as indicated)
