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FIGURE 1.4
Verification of identified LDproteins. (A-D) The same quantity of proteins fromLDs, membranes
(Mem), Cyto, and cell lysates (CL) is separated by SDS-PAGE and subjected to Western
blottingusinganti-ro05869(A), anti-ro04894 (B), anti-ro00061 (C), anti-ro02258, anti-ro06757,
anti-ro01247, anti-ro02146, anti-ro06190, or anti-ro02104 (D). (E) MLDS (ro02104)
deletionmutant cells of RHA 1 are used for overexpression of GFP vector plasmid (a-1-a-4) and
full length MLDS (b-1-b-4). The cells are then visualized by confocal microscopy. Bar
m.
(F) A transgenic strain with pvha-6::dhs-3::gfp (single copy) is generated to represent DHS-3
location in wild-type C. elegans. The image is taken using confocal microscopy. Bar
¼
5
m
m.
(A-E) are reproduced from Ding et al. (2012) .
¼
5
m
1.4 DISCUSSION
Recent LD proteomic studies have provided many useful clues to understand the bi-
ological role of LDs as a cellular organelle. Several groups of functional proteins
have identified, including lipid synthetic enzymes, membrane traffic proteins, sig-
naling proteins, and protein degradation mediators ( Yang et al., 2012 ). Recently,
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