Biology Reference
In-Depth Information
(
Yen et al., 2009
), using a dual tracer method (
Buhman et al., 2002
), and/or using
behenic acid as a nonabsorbable lipid marker (
Jandacek, Heubi, & Tso, 2004
).
CARS imaging is a unique technology for imaging intestinal CLDs. In combina-
tion with biochemical and physiological methods for assessing dietary fat absorption,
this technique provides a powerful and efficient method to visualize and quantify
CLDs. As mentioned throughout this protocol, the CLDs in the intestine are
constantly changing with time after a meal or gavage, meal or diet composition,
and location of the intestine sampled. The protocols outlined will serve to maximally
interpret and provide reliable results in capturing the dynamic process of dietary fat
absorption.
Acknowledgment
This work was supported by AHA NCRP Scientist Development Grant 0835203 N to K. K. B.
and an AHA Predoctoral Fellowship 11PRE5140017 to A. U.
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