Biology Reference
In-Depth Information
CHAPTER
9
Imaging Cytoplasmic Lipid
Droplets in Enterocytes and
Assessing Dietary Fat
Absorption
Aki Uchida
*
,{
, Hyeon Jeong Lee
{,{
, Ji-Xin Cheng
{,{,}
, and Kimberly K. Buhman
*
*
Department of Nutrition Science, Purdue University, West Lafayette, Indiana, USA
{
Interdisciplinary Life Science Program, Purdue University, West Lafayette, Indiana, USA
{
Department of Chemistry, Purdue University, West Lafayette, Indiana, USA
}
Weldon School of Biomedical Engineering, Purdue University, West Lafayette, Indiana, USA
CHAPTER OUTLINE
Introduction and Rationale....................................................................................... 152
9.1 Materials and Methods..................................................................................... 153
9.1.1 Collection of Intestine Tissue from Mice for Analysis ........................ 153
9.1.2 Imaging CLDs in Enterocytes .......................................................... 154
9.1.2.1 Coherent Anti-Stokes Raman Scattering (CARS) Microscopy.....
154
9.1.2.2 Oil Red O ................................................................................
156
9.1.2.3 Results....................................................................................
157
9.1.3 Assessing Dietary Fat Absorption .................................................... 158
9.1.3.1 Biochemical TAG Quantification in Intestinal Mucosa................
158
9.1.3.2 Dietary Fat Absorption—Assessed by Percent Lipid in Feces ....
160
9.1.3.3 Blood TAG in Response to Dietary Fat......................................
161
9.2 Discussion....................................................................................................... 162
Acknowledgment..................................................................................................... 165
References ............................................................................................................. 165
Abstract
The primary function of the small intestine is digesting and absorbing nutrients from
consumed food. Because of this, the small intestine is often thought of as a nutrient
thoroughfare—enterocytes taking up nutrients on the apical side and then secreting
nutrients from the basolateral side. The small intestine is not commonly thought of as
a lipid storage organ; however, when meals and diets containing high amounts of
