Biology Reference
In-Depth Information
CHAPTER
8
Hong Wang
*
, Ming Lei
{
, Ru-ching Hsia
{
,1
, and Carole Sztalryd
*
,},1
Analysis of Lipid Droplets
in Cardiac Muscle
*
Division of Endocrinology, Department of Medicine, School of Medicine, University of Maryland,
Baltimore, Maryland, USA
{
Visualization Sciences Group, An FEI Company, Burlington, Massachusetts, USA
{
Core Imaging Facility, Dental School, University of Maryland, Baltimore, Maryland, USA
}
Geriatric Research, Education, and Clinical Center, Baltimore Veterans Affairs Health Care
Center, Baltimore, Maryland, USA
CHAPTER OUTLINE
Introduction and Rationale....................................................................................... 130
8.1 Isolation of Cardiac LD Proteins and Preparation of Cardiac LDs for Analysis
by Western Blot ............................................................................................... 132
8.1.1 Materials ....................................................................................... 133
8.1.1.1 Reagents................................................................................. 133
8.1.1.2 Buffers .................................................................................... 134
8.1.2 Equipment..................................................................................... 134
8.1.3 Experimental Design....................................................................... 134
8.1.4 Analysis of LDs by Western Blot....................................................... 135
8.1.5 Results and Discussion ................................................................... 136
8.2 Analysis of Cardiac LDs by 2D Analysis of LDs by Conventional TEM ................... 137
8.2.1 Materials ....................................................................................... 138
8.2.1.1 Reagents and Buffers .............................................................. 138
8.2.2 Equipment..................................................................................... 138
8.2.3 Experimental Design....................................................................... 138
8.2.4 Results and Discussion ................................................................... 140
8.3 Progress in 3D EM Technology May Provide New Tools to Study Cardiac LDs
and Mitochondria Interactions .......................................................................... 142
Conclusions............................................................................................................ 145
Acknowledgments ................................................................................................... 146
References ............................................................................................................. 146
1
These authors contributed equally to this work.
