Biology Reference
In-Depth Information
CHAPTER
From Molecules to Mice
23
Kim C. Jonas
*
, Adolfo Rivero-M ยจ ller
{
, Ilpo T. Huhtaniemi
*
,{
,
and Aylin C. Hanyaloglu
*
*
Department of Surgery and Cancer, Institute of Reproductive and Developmental Biology,
Imperial College London, London, United Kingdom
{
Department of Physiology, Institute of Biomedicine, University of Turku, Turku, Finland
G Protein-Coupled
Receptor Transactivation:
CHAPTER OUTLINE
Introduction ............................................................................................................ 434
23.1 Materials........................................................................................................435
23.2 Methods .........................................................................................................436
23.2.1 Protein-Protein Interactions ........................................................ 437
23.2.2 Functional Measurement of Receptor Transactivation in Vitro ......... 437
23.2.3 Studying Transactivation in Vivo .................................................. 440
23.2.3.1 Generation of BAC Constructs............................................ 440
23.2.3.2 Embryo Generation, Detection of BAC Transgenic
Offspring, and Breeding Strategy ...................................................... 442
23.2.3.3 Analysis of Male Reproductive Tract and Testes ................. 442
23.2.3.4 Fertility .............................................................................. 446
23.3 Discussion......................................................................................................447
Summary ................................................................................................................ 448
Acknowledgments ................................................................................................... 448
References ............................................................................................................. 448
Abstract
G protein-coupled receptors (GPCRs) mediate a diverse range of physiological func-
tions via activation of complex signaling systems. Organization of GPCRs in to di-
mers and oligomers provides a mechanism for both signal diversity and specificity in
cellular responses, yet our understanding of the physiological significance of dimer-
ization, particularly homodimerization, has not been forthcoming. This chapter will
describe how we have investigated the physiological importance of GPCR homodi-
merization, using the luteinizing hormone/chorionic gonadotropin receptor as a
