Biology Reference
In-Depth Information
Microdomains
n
-Dodecyl
-
D
-maltoside (DDM)
Ammonium persulfate
Glycerol
TEMED
ddH
2
O
b
22.4.1.2
Equipment
Gradient former
Peristaltic pump
Gel electrophoresis apparatus
22.4.1.3
Buffers and solutions
3
Gel buffer (1.5 M aminocaproic acid, 150 mM Bis-tris, pH 7.0).
19.68 g aminocaproic acid
3.14 g Bis-tris
Up to 100 mL ddH
2
O
Cathode buffer (15 mM Bis-tris, 50 mM tricine, pH 7.0).
3.14 g Bis-tris
8.96 g tricine
Up to 1 L ddH
2
O
Blue cathode buffer.
100 mL cathode buffer
0.02 g Serva Blue G
Anode buffer (50 mM Bis-tris, pH 7.0).
20.93 g Bis-tris
Up to 2 L ddH
2
O
Acrylamide/bisacrylamide mix (48% acrylamide and 1.5% bisacrylamide
(99.5T,C)).
24.0 g acrylamide
0.75 g bisacrylamide
Up to 50 mL ddH
2
O
Membrane buffer.
0.5 mL 3
gel buffer
0.5 mL 2 M aminocaproic acid
L 500 mM EDTA
SBG sample buffer (0.75 M aminocaproic acid, 5% Serva Blue G).
3.75 mL 2 M aminocaproic acid
0.5 g Serva Blue G
Up to 10 mL ddH
2
O
4
m
2 M aminocaproic acid.
13.12 g aminocaproic acid
50 mL ddH
2
O
10% DDM (0.1 g/mL).
