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FIGURE 19.3
Number and brightness analysis of raster-scanned images of giant unilamellar vesicle (GUV)
tubulation by ENTH domain. (A) The time course of the relative abundance of different
aggregates for epsin 1 ENTH WT. (B) The time course of the relative abundance of
different aggregates for epsin ENTH K23E/E42K mutant. (C) A representative image of POPC/
POPE/POPS/PtdIns(4,5)P 2 /Rh-PE GUV shown by Rh-PE fluorescence after a 10-min
treatment with epsin 1 ENTH WT. (D) Distribution of ENTH WT aggregates (monomer to
20-mer) was superimposed onto the image of GUV. (E) Distribution of ENTH WT aggregates
Continued
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