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FIGURE 16.3
(A) Model for the clustering of FKBP-mGFP constructs when AP2018 is added. (B) Steady-
state fluorescence images and anisotropy images of FKBP-mGFP constructs.
Results adapted from Bader et al. (2009) and Hofman et al. (2010) .
e 2 ot þ
r inf (16.5)
The limiting anisotropy r inf is usually reached within nanoseconds ( Fig. 16.4 ). At r inf ,
homo-FRET has occurred multiple times and the probability of emitting a photon is
equal for all fluorophores.
In this case, the limiting anisotropy is a direct measure of the cluster size that is
not affected by the energy transfer rate
r homo FRET t
ðÞ¼
ð
r mono
r inf
Þ
o
, and it can be determined using Eq. (16.6)
( Bader et al., 2007 ):
r mono
N þ
r et N
1
r inf ¼
(16.6)
N
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