Biology Reference
In-Depth Information
FIGURE 16.3
(A) Model for the clustering of FKBP-mGFP constructs when AP2018 is added. (B) Steady-
state fluorescence images and anisotropy images of FKBP-mGFP constructs.
Results adapted from
Bader et al. (2009)
and
Hofman et al. (2010)
.
e
2
ot
þ
r
inf
(16.5)
The limiting anisotropy
r
inf
is usually reached within nanoseconds (
Fig. 16.4
). At
r
inf
,
homo-FRET has occurred multiple times and the probability of emitting a photon is
equal for all fluorophores.
In this case, the limiting anisotropy is a direct measure of the cluster size that is
not affected by the energy transfer rate
r
homo
FRET
t
ðÞ¼
ð
r
mono
r
inf
Þ
o
, and it can be determined using Eq.
(16.6)
(
Bader et al., 2007
):
r
mono
N
þ
r
et
N
1
r
inf
¼
(16.6)
N