Biology Reference
In-Depth Information
Zeba 5 mL, 7 kDa MWCO spin columns
Slide-A-Lyzer 10 kDa MWCO 0.5-3 mL dialysis unit
Sodium periodate (MW
¼
213.89 g/mol)
Sodium sulfite (MW
126.04 g/mol)
Sodium cyanoborohydride (MW
¼
62.84 g/mol)
Biocytin hydrazide (Pierce 28020; MW
¼
¼
368.20 g/mol)
BupH phosphate 7.2 (Pierce 28372)
Glycerol
1D4 mAb (10 mg/mL in PBS with 10% glycerol 0.02% NaN
3
, stored at
80
C)
15.5.1.2
Solutions and buffers
10
PBS:
Dissolve one pack BupH phosphate in purified water to give 50 mL total volume
(1000 mM Na phosphate pH 7.2, 1500 mM NaCl). Filter with 0.2
m
m Steriflip
filtration unit.
PBS:
5mL10
PBS.
45 mL purified water.
Filter and degas with 0.2
m
m Steriflip filtration unit.
10% NaN
3
:
100 mg sodium azide
0.9 mL purified water
350 mM NaIO
4
:
75 mg sodium metaperiodate.
1 mL purified water.
Prepare solution directly before use and protect from light.
400 mM Na
2
SO
3
:
50 mg sodium sulfite.
1 mL purified water.
Prepare directly before use.
50 mM BIOCYTIN HYDRAZIDE:
18.41 mg biocytin hydrazide
1 mL DMSO
300 mM CYANOBOROHYRIDE:
18.9 mg sodium cyanoborohydride.
1 mL PBS.
Prepare directly before use.
