Biology Reference
In-Depth Information
Application of FRET and Microscopy
A: Zeiss LSM780
B: Spinning-Disk
C: Wide-field
D: TIRF
0.2
Ef
D
0.1
0.0
FIGURE 14.9
Interaction of 5-HT
1A
and 5-HT
7
receptors investigated by lux-FRET. Images of apparent
FRET efficiency Ef
D
in an N1E-115 cell coexpressing 5-HT
1A
-CFP and 5-HT
7
-YFP receptors
were created according to the lux-FRET method measured at (A) Zeiss LSM 780 and
(B) spinning disk microscope, and cell coexpressing 5-HT
1A
-YFP and 5-HT
7
-CFP receptors
recorded using the iMIC platform in (C) Wide-field and (D) TIRF mode. Scale bar: 10
m
m.
Acknowledgments
This study was supported by the Federal Ministry of Education and Research of the Federal
Republic of Germany (0315690D to S.P. and A.Z.). E.G.P. was supported by the Deutsche
Forschungsgemeinschaft (grants PO732 and SFB621/C12).
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