Biology Reference
In-Depth Information
FIGURE 14.4
Lux-FRET acquisition protocols at Zeiss LSM 780. (A) Standard channel mode configuration with defined two excitation tracks with a constant
binning of two PMT channels to each channel. (B) Application of the LIC Macro toolbox to improve spectral detection capabilities for lux-FRET. (C)
LICMacro enables us to use a multitrack lambda mode with sequential line-wise switching of alternating excitation wavelength. Sample recording is
shown with three lambda track mode.
