Biology Reference
In-Depth Information
Application of FRET and Microscopy
Triton X-100 solution (Sigma, cat.no.93427)
D
glucose (Sigma, cat.no.G7021)
NaHPO
4
(Roth, cat.no.P030.1)
KH
2
PO
4
(Roth, cat.no.P018.1)
Ethanol—99.8% (Roth, cat.no.90654)
Acetone—99.5% (Roth, cat.no.5025.2)
Methanol—99% (Roth, cat.no.8388.5)
Double-distilled water
þ
Cell culture media and reagents
Dulbecco's modified Eagle's medium—high glucose (DMEM) (Sigma Aldrich,
cat.no.D5648-10X)
Fetal bovine serum (Biochrom, cat.no.S 0415)
Penicillin-streptomycin (Invitrogen, cat.no.15070-063)
NaCl (Roth, cat.no.3957.5)
NaHCO
3
(Roth, cat.no.8551.1)
KCl (Roth, cat.no.6781.1)
MgCl
2
hexahydrate (Roth, cat.no.2189.1)
CaCl
2
dihydrate (Roth, cat.no.5239.1)
HEPES (Roth, cat.no.9105.3)
NaOH—32% solution (Roth, cat.no.T197.1)
Dimethyl sulfoxide (DMSO) (Sigma Aldrich, cat.no.472301)
Poly-
L
-lysine (PLL) (Sigma Aldrich, cat.no.P2636-25)
Opti-MEM reduced-serum medium (1
), liquid (Life Technologies,
cat.no.11058021)
Cell culture petri plates (10 cm) (Nunc, cat.no.150350)
6-well cell culture plate (Greiner, cat.no.657160)
12-well cell culture plate (Greiner, cat.no.665180)
18 mm (thickness 0.177 mm), 50 mm (Thermo Scientific, cat.no.004710482,
004711182), and 24
24 mm (Roth, cat.no.H 875) glass coverslips
Amino acids and organic compounds for medium and protease inhibitors
L
-Cystine (Sigma, cat.no.C7352-25G)
L
-Alanine (Sigma, cat.no.A7469-25G)
L
-Aspartic acid (Sigma, cat.no.A4534-100G)
L
-Proline (Sigma, cat.no.P5607-25G)
L
-Glutamic acid monosodium salt hydrate (Sigma, cat.no.G5889-100G)
L
-Asparagine monohydrate (Sigma, cat.no.A4284-100G)
Leupeptin (Roth, cat.no.CN33.1)
Chymostatin (Sigma, cat.no.C7268-5MG)
Antipain (Roth, cat.no.2933.1)
Pepstatin (Roth, cat.no.2936.1)