Biology Reference
In-Depth Information
Application of FRET and Microscopy
￿ Triton X-100 solution (Sigma, cat.no.93427)
￿D
glucose (Sigma, cat.no.G7021)
￿ NaHPO 4 (Roth, cat.no.P030.1)
￿KH 2 PO 4 (Roth, cat.no.P018.1)
￿ Ethanol—99.8% (Roth, cat.no.90654)
￿ Acetone—99.5% (Roth, cat.no.5025.2)
￿ Methanol—99% (Roth, cat.no.8388.5)
￿ Double-distilled water
þ
Cell culture media and reagents
￿ Dulbecco's modified Eagle's medium—high glucose (DMEM) (Sigma Aldrich,
cat.no.D5648-10X)
￿ Fetal bovine serum (Biochrom, cat.no.S 0415)
￿ Penicillin-streptomycin (Invitrogen, cat.no.15070-063)
￿ NaCl (Roth, cat.no.3957.5)
￿ NaHCO 3 (Roth, cat.no.8551.1)
￿ KCl (Roth, cat.no.6781.1)
￿ MgCl 2 hexahydrate (Roth, cat.no.2189.1)
￿ CaCl 2 dihydrate (Roth, cat.no.5239.1)
￿ HEPES (Roth, cat.no.9105.3)
￿ NaOH—32% solution (Roth, cat.no.T197.1)
￿ Dimethyl sulfoxide (DMSO) (Sigma Aldrich, cat.no.472301)
￿ Poly- L -lysine (PLL) (Sigma Aldrich, cat.no.P2636-25)
￿ Opti-MEM reduced-serum medium (1
), liquid (Life Technologies,
cat.no.11058021)
￿ Cell culture petri plates (10 cm) (Nunc, cat.no.150350)
￿
6-well cell culture plate (Greiner, cat.no.657160)
￿
12-well cell culture plate (Greiner, cat.no.665180)
￿
18 mm (thickness 0.177 mm), 50 mm (Thermo Scientific, cat.no.004710482,
004711182), and 24
24 mm (Roth, cat.no.H 875) glass coverslips
Amino acids and organic compounds for medium and protease inhibitors
￿ L -Cystine (Sigma, cat.no.C7352-25G)
￿ L -Alanine (Sigma, cat.no.A7469-25G)
￿ L -Aspartic acid (Sigma, cat.no.A4534-100G)
￿ L -Proline (Sigma, cat.no.P5607-25G)
￿ L -Glutamic acid monosodium salt hydrate (Sigma, cat.no.G5889-100G)
￿ L -Asparagine monohydrate (Sigma, cat.no.A4284-100G)
￿ Leupeptin (Roth, cat.no.CN33.1)
￿ Chymostatin (Sigma, cat.no.C7268-5MG)
￿ Antipain (Roth, cat.no.2933.1)
￿ Pepstatin (Roth, cat.no.2936.1)
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