Biology Reference
In-Depth Information
0.03
*
0.02
***
***
0.01
***
***
0
0
0.5
1
1.5
2
A
1
R (not fused to BRET
2
probes)
plasmid (
m
g)
B
0.03
*
0.02
**
***
***
0.01
***
0
0
0.5
1
1.5
2
TP
a
(not fused to BRET
2
probes)
plasmid (
m
g)
C
0.05
0.04
0.03
0.02
0.01
0
0
1
2
3
4
LPA1R (not fused to BRET
2
probes)
plasmid (
m
g)
FIGURE 12.2
The competitive BRET
2
assay for GPCR oligomerization. HEK293T cells were cotransfected
with a fixed concentration of HA-A
1
R-Rluc and HA-A
1
R-GFP
2
plasmids and increasing
amounts of HA-A
1
R (A), HA-TP
a
(B), or HA-LPA1R (C) plasmids. Data represent the
mean
SEM for three independent experiments. *P
<
0.05, **P
<
0.005, ***P
<
0.001
