Biology Reference
In-Depth Information
1.06
1.06
H 1 R-mTq2
H 1 R-sYFP2
1.02
1.02
0.98
0.98
1.0
10
100
1000
1.0
10
100
1000
t (ms)
t (ms)
1.0030
cross-correlation
1.0015
1.0000
1.0
10
100
1000
t (ms)
FIGURE 11.3
Typical auto- and cross-correlation curves for HeLa cells cotransfected with H 1 R-
mTurquoise2 and H 1 R-sYFP2. The curves were fitted (black lines) to models including terms
for diffusion, dark-state kinetics, and the presence of monomers and dimers.
cross-correlated using multi-tau algorithms ( Wahl, Gregor, Patting, & Enderlein,
2003 ) resulting in the two autocorrelation curves G CC ( t ) (for the CFP channel)
and G YY ( t ) (for the YFP channel) and the cross-correlation curve G CY ( t ).
Figure 11.3 shows some typical experimental curves.
The two autocorrelation and cross-correlation curves are fitted according to
Eq. (11.5) . Since we define the direction of line scanning to be the x -axis, so perpen-
dicular to the membrane, diffusion along the x -axis can be neglected due to the lim-
ited thickness of the plasma membrane (3-10 nm) in relation to the e 2 radius of the
detection volume (
250 nm).
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