Biology Reference
In-Depth Information
100
Vehicle, t 1/2 = 2.55 min
PDC113.824,
80
t 1/2 = 1.55 min
60
40
20
0
0
5
10
15
20
25
30
PGF2
α
(min)
FIGURE 9.3
Allosteric modulation of orthosteric ligand binding to FP by a small molecule allosteric
modulator. Dissociation kinetics of [ 3 H]-PGF2a from FP in the presence of a vehicle or 2 mM
PDC113.284 (a small molecule allosteric modulator). Data are representative of three
independent experiments.
100
80
FP,
t 1/2 = 3.40 min
FP + AT1R,
t 1/2 = 5.13 min
60
40
20
0
0
5
10
15
20
25
30
PGF2
α
(min)
FIGURE 9.4
Allosteric modulation of orthosteric ligand binding to FP via dimerization with another GPCR,
AT1R. Dissociation kinetics of (A) [ 3 H]-PGF2a binding from cells expressing FP alone or
in the presence of the angiotensin II (Ang II) type I receptor (AT1R). Data are representative
of four independent experiments.
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