Biology Reference
In-Depth Information
19.
TBST: TBS (20 mM Tris/HCl, pH
¼
7.4; 150 mM NaCl) and 0.25% Tween-20.
20.
TBST-milk: TBST, 5% milk.
21.
TBST-BSA: TBST, 5% BSA (Albumin from bovine serum, Sigma, #A2153).
22.
Ms anti-Myc antibody: mouse anti-c-Myc antibody, Sigma-Aldrich 9E10,
#M4439.
23.
Rb anti-Myc antibody: rabbit anti-c-Myc antibody, Santa Cruz Biotechnology,
sc789, #D1008.
24.
Ms anti-HA antibody: mouse anti-HA antibody, Covance, MMS-101P,
#E11AF0013.
25.
Rb anti-HA antibody: rabbit anti-HA antibody, Life Technologies, #71-5500.
26.
Antimouse antibody conjugated with HRP: ECL Mouse IgG, HRP-linked
whole A
, GE Healthcare; #NA931.
27.
GAR—red: Alexa Fluor
®
594 Goat Antirabbit IgG, Life Technologies,
#A-11012.
28.
GAM—green: Alexa Fluor
®
488 Goat Antimouse IgG, Life Technologies,
#A-11001.
29.
Pierce ECL Western Blotting Substrate: Thermo Scientific, #32209.
30.
DSP: dithiobis[succinimidyl propionate], Thermo Scientific, #22586. Fist
dilution of DSP at 25 mM in DMSO, then final dilution at 1.25 mM in PBS.
31.
Phosphatase inhibitors: 10 mM NaF, 2 mM Na
þ
vanadate, and 1 mM Na
þ
pyrophosphate.
32.
DDM:
n
-dodecyl
b
-
D
-maltoside, Sigma, #D5172.
33.
DDM lysis buffer: for 10 ml of buffer, 40 mg of DDM, 20 mM HEPES,
150 mM NaCl, 1% NP40, 10% glycerol, phosphatase inhibitors, and half a
tablet of protease inhibitors, adjust the volume with water.
34.
Anti-HA/agarose beads: mouse anti-HA-tag monoclonal antibody,
agarose-conjugated, Sigma, #DMAB8895.
35.
Glass slides and coverslips (
b
18 mm).
∅
36.
PORN 1
: poly-
L
-ornithine hydrobromide (10 mg/L in PBS), Sigma,
#P3655.
37.
PFA: paraformaldehyde 16% in water, Euromedex, #RT 15710-S.
38.
PBS-glycine: PBS, 0.1 M glycine (Sigma, #G7126).
39.
PBS-Triton: PBS, 0.05% Triton X-100 (Sigma, #T8532).
40.
PBS-gelatin: PBS, 0.2% gelatin (Sigma, #G9391).
41.
5-HT: 5-hydroxytryptamine (serotonin creatinine sulfate monohydrate, Sigma,
#H7752).
42.
BIMU 8: 2,3-dihydro-N-[(3-endo)-8-methyl-8-azabicyclo[3.2.1]oct-3-yl]-3-
(1-methylethyl)-2-oxo-1H-benzimidazole-1-carboxamide hydrochloride,
Tocris Bioscience, #4374.
43.
VECTASHIELD: VECTASHIELD Mounting Medium, Vector Laboratories,
#H-1000.
44.
HBS: 20 mM HEPES, 150 mM NaCl, 4.2 mM KCl, 0.9 mM CaCl
2
, 0.5 mM
MgCl
2
, 0.1% glucose, and 0.1% BSA.
45.
HBS-KF: HSB, 200 mM KF.
