Biology Reference
In-Depth Information
CHAPTER
7
Sylvie Claeysen
*
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, Romain Donneger
*
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, Patrizia Giannoni
*
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,
Florence Gaven
*
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, and Lucie P. Pellissier
*
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*
CNRS, UMR-5203, Institut de Ge´nomique Fonctionnelle, Montpellier, France
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Inserm, U661, Montpellier, France
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Universite´s de Montpellier 1 & 2, UMR-5203, Montpellier, France
Serotonin Type 4 Receptor
Dimers
CHAPTER OUTLINE
Introduction ............................................................................................................ 124
7.1 Materials........................................................................................................ 125
7.2 Methods ......................................................................................................... 127
7.2.1 Cell Transfection ..........................................................................127
7.2.2 Detection of 5-HT
4
R Dimersx by Western Blot ................................127
7.2.2.1 Cell Lysate and Membrane Preparation ..................................
127
7.2.2.2 Solubilization and Deglycosylation of the Samples...................
129
129
7.2.3 Detection of 5-HT
4
R Dimers by Coimmunoprecipitation...................131
7.2.4 Analysis of 5-HT
4
R Dimers by Immunofluorescence ........................131
7.2.5 Analysis of 5-HT
4
R Dimers by TR-FRET .........................................134
7.3 Discussion...................................................................................................... 136
Summary ................................................................................................................ 137
Acknowledgments ................................................................................................... 137
References ............................................................................................................. 138
7.2.2.3 Western Blot and Detection of the 5-HT
4
Receptor Dimers .....
Abstract
Numerous class A G protein-coupled receptors and especially biogenic amine recep-
tors have been reported to form homodimers. Indeed, the dimerization process might
occur for all the metabotropic serotonergic receptors. Moreover, dimerization
appears to be essential for the function of serotonin type 2C (5-HT
2C
) and type
