Biochemical and Imaging Methods to Study Receptor Membrane Organization and Association with Lipid Rafts - Methods in Cell Biology - page 113

FIGURE 6.2
Superresolution techniques map protein distribution and clustering on the cell membrane.
(A) Single-molecule PALM images of the raftophilic protein Lck. (B) Protein cluster maps
generated from local point-pattern analysis of outline region. (C) Maps of Lck clusters and
molecules inside clusters. Adapted from Rossy et al. (2013) . (D-E) Confocal and STED
images of the pathogen recognition receptor Dendritic Cell-Specific Intercellular adhesion
molecule-3-Grabbing Non-integrin (DC-SIGN). (F) Normalized intensity distribution of
fluorescent spots obtained by STED for DC-SIGN and a mutant ( Rep) compared to the

intensity of spots found on glass. Adapted from Manzo et al. (2012) . (G) Confocal versus
NSOM image of cholera toxin-G M1 clusters at the cell membrane. (H) Dual-color NSOM of
CD55 (green) and CTxB-GM1 nanodomains (red). (I) Nearest-neighbor distributions analysis
of CD55 to its closest CTxB-GM1 nanodomain (bars) compared to simulations of random
spatial distribution of proteins and CTxB-GM1 clusters.

D

Adapted from van Zanten et al. (2010) .