Biology Reference
In-Depth Information
CHAPTER
1
Spatial Intensity Distribution
Analysis (SpIDA): A New
Tool for Receptor Tyrosine
Kinase Activation and
Transactivation
Quantification
Annie Barbeau
*
,{
, Jody L. Swift
{
, Antoine G. Godin
},}
, Yves De Koninck
*
,{
,
Paul W. Wiseman
||
, and Jean-Martin Beaulieu
*
,{
*
Department of Psychiatry and Neuroscience, Faculty of Medicine,
Laval University, Que´bec, Canada
{
Institut universitaire en sante´ mentale de Que´bec, Que´bec, Canada
{
Department of Medicine, The University of British Columbia, Vancouver,
British Columbia, Canada
}
University of Bordeaux, LP2N, Talence, France
}
CNRS & Institut d'Optique, LP2N, Talence, France
||
Departments of Chemistry and Physics, McGill University, Montreal, Que´bec, Canada
CHAPTER OUTLINE
Introduction ................................................................................................................ 2
1.1 Theory of Spatial Intensity Distribution Analysis..................................................... 4
1.1.1 Theoretical Basis of SpIDA.................................................................. 4
1.2 SpIDA: Examples of Application to RTK.................................................................. 4
1.2.1 Quantification of EGFR-eGFP.............................................................. 4
1.2.2 Application to RTK Transactivation in a Native System: Neurons
expressing Dopamine Receptors........................................................... 6
1.3 Procedure for SpIDA............................................................................................. 6
1.3.1 Material and Apparatus ....................................................................... 6
1.3.1.1 Sample Preparation ......................................................................8
1.3.2 Necessary Experimental Controls: Defining the QB for Monomeric
moiety ............................................................................................... 9
1.3.2.1 Determining the Monomeric QB for Receptors Tagged
with Fluorescent Proteins ..........................................................................9
