Biomedical Engineering Reference
In-Depth Information
FIGURE 2.5
Laser scanning confocal microscope (LSCM) images of MSC grown in
epidermal induction medium on Coll/PLLCL nanofibers expressing Ker 10 (a), filaggrin
(b), and involucrin (c). Double staining for Ker 10 (d) and filaggrin (e) and (f) overlay image of
(d) and (e) after 15 days of cell culture. Reproduced with permission from Ref. [57].
found on the Coll/PLACL nanofibrous scaffolds. The study suggest that the electrospun
Coll/PLACL nanofibers supported the differentiation of MSCs in the presence of
growth factors, thereby creating the possibility of cell-scaffold transplantation of a
construct with differentiated keratinocytes to the sites of skin injury. Kobayashi and
Spector (2009) investigated the clinical effects of mechanical stress on the behavior of
BM-MSCs in a collagen type I/glycosaminoglycan scaffold matrix for 1 week under
cyclic stretch loading conditions.
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Their results suggested that mechanical stress
may affect the proliferation and differentiation of stem cells and, subsequently, the
wound healing process via interactions between the stem cells and scaffold matrix.
Adipose-derived stem cells (ADSCs) secrete various growth factors that control
and manage damaged neighboring cells, and this has been identified as an essential
function of ADSCs. ADSCs stimulated both collagen synthesis and migration of
dermal fibroblasts, which improved wrinkling and accelerated wound healing in
animal models.
59,60
Novel cost-effective electrospun nanofibrous scaffolds are established for wound
dressing and allogeneic cultured dermal substitute through the cultivation of human
dermal fibroblast for skin defects. A combination of growth factors together with the
porous structure of the scaffolds might substantially improve the skin regeneration
efficacy. This can be achieved by a simple incorporation of growth factors during the
scaffold preparation, either with an electrospinning process or obtaining a controlled
release of growth factors via a coaxial electrospinning technique.
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